GM8 MicroStat - offers a simple, fast and flexible method for determination of key parameters in fermentation broths, cell culture media and other microbial applications. Assays include: Glucose, Lactate, Ethanol, Methanol, Ammonia, Glycerol, Sucrose, Lactose
and Glutamine.

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Analytical Principle:

In oxidase enzyme reactions, the analyser measures the rate of oxygen uptake and under appropriate controlled conditions, this is directly proportional to the concentration of the analyte.

Example: Lactate

Substrates for dehydrogenase enzymes are measured using a pre-reaction in which the oxidation of NADH (produced or consumed) is then monitored.

Example: Ammonia

Under the conditions of the assay, the rate of oxidation of excess NADH by peroxidase (POD) is inversely proportional to ammonia concentration.

Operation:

For many assays, the simple injection of a sample is all that is needed to obtain a result and prepare the analyser for the next analysis. Sample injection via an accurate positive displacement pipette triggers the complete analytical cycle and a hard-copy result is then printed within 20 seconds. The analyser is first calibrated with a standard of the metabolite in question.
Samples can be any aqueous solution (pH 5-7), culture fluid, etc. Turbidity or opacity is no problem.
Analyses are menu-driven via the 32-character display which guides the operator through the complete procedure. Subsidiary menus are reached via the keypad which enables the user to change analysis type, optimise operational modes, utilise special functions and perform statistical data analysis. The display also provides self-test diagnostics in relation to electrode status and fluid movements. An RS232 output is available for system integration.
Reagent changeover between analytes is performed quickly and simply and all fluid pathways inside the analyser can be rapidly sterilised without compromising performance.

Typical Analytical Performance
Industrial L-Glutamine Assay:

Injection Volume

25µl

Analytical Range

0.5 - 20mmol/L

Precision

Typical within-run CV = 1-2% at 8.0mmol/L

Detection Limit

0.2mmol/L

Sensitivity (Analyser)

1µmol/L

Reagent Stability

9 months unopened when stored at 0-5°C
7-10 days once reconstituted stored at 0-5°C

Instrument Specifications
 

Method
Sensor
Reaction temperature
Display
Printer

Enzymatic oxygen-rate
Clark-type amperometric oxygen electrode
30°C
32 character backlit LCD
16 column dot matrix, 1 line/sec 

Statistical programmes

a)Sequential, giving mean, S.D and C.V.
b) Selected results for 2 analytes, retains up to 75 entries

Interface
Power
Dimensions
Weight

Serial data port, optional Windows software available
100-250V AC, 50-60Hz, 12-15V DC, 60VA
27cm (width) x 35cm (depth) x 25cm (height)
5.8Kg

Ordering Information
 

GM8 MicroStat
GMRD-002A
GMRD-015

Analyser, mains version
Glucose oxidase reagent, 360 analyser cycles
Glucose standard, 1%W/V, 30ml (other standards available)

GMRD-090/093/092

Lactate reagent for 100, 250 and 1000 analyser
cycles respectively, incl. standard

GMRD-110I
GMRD-110(5)
GMRD-125
GMRD-123A
GMRD-151
GMRD-152
GMRD-185

Alcohol reagent kit, 70 analyser cycles
Alcohol standard, 5%V/V, 10ml (other values available)
Methanol reagent kit, 70 analyser cycles
Methanol standard, 120mg/L (other standards available)
L-Glutamine/Ammonia reagent kit, 60 tests, incl. standard
Ammonia reagent kit, 60 tests, incl. standard
Glycerol reagent kit, 140 cycles, incl. standard

Other reagent kits, standards and accessories are available
please contact Analox for details