P-GM7 MicroStat
Top-of-the-range sports analyser, with pre-programmed assays for a detailed blood profile of the competitor.
Assays include: Glucose, Lactate, Cholesterol, Alcohol, Urate, Pyruvate Glutamine, Ammonia

P-GM7


The integral rechargeable battery allows the analyser to be used at track or poolside and an optional all-weather carrying case enables the P-GM7 to be operated in virtually any environment.

The Model P-GM7 is ideal for routine training monitoring, sports science teaching courses and research projects. A mains version, Model GM7, is also available.

Analytical Principle:

    In oxidase enzyme reactions, the analyser measures the rate of oxygen uptake and under appropriate controlled conditions, this is directly proportional to analyte concentration.

      Example:
      Lactate in whole blood or plasma

    Substrates for dehydrogenase enzymes are measured using two stage reactions in which the oxidation of NADH (produced or consumed) is monitored.

      Example:
      Plasma Ammonia:
      Ammonia is converted to L-glutamate by a-ketoglutarate in the presence of glutamate dehydrogenase (GLDH) and excess NADH:

    Under the conditions of the assay, the rate of oxidation of excess NADH by peroxidase (POD) is inversely proportional to ammonia concentration.

Operation:

    For many assays, the simple injection of a sample is all that is needed to obtain a result and prepare the analyser for the next analysis. Sample injection via an accurate positive displacement pipette triggers the complete analytical cycle and a hard-copy result is printed within 20 seconds. The analyser is first calibrated with a standard of the metabolite in question.

    Samples can be whole blood (e.g. key assays such as lactate, glucose, urea), plasma, serum or other biological fluids.

    Analyses are menu-driven via the 32-character display which guides the operator through the complete procedure. Subsidiary menus are reached via the keypad which enables the user to change analysis type, optimise operational modes, utilise special functions and perform statistical data analysis. The display also provides self-test diagnostics in relation to electrode status and fluid movements.

    Changeover between analytes is quickly and simply effected and all fluid pathways inside the analyser can be rapidly sterilised without compromising performance.

 

Analytical Performance

 

Glucose

Lactate

Alcohol

Precision
(within run)

Plasma; n=20, CV=1.4%
@ 10.0mmol/L
Whole blood; n=40, CV=0.85%
@ 12.0mmol/L

Plasma; n=30, CV=0.6%
@ 10.0mmol/L
Whole blood; n=22, CV=1.6%
@ 5.0mmol/L

Plasma; CV=1-2%
@ 100md/dl
Whole blood; CV=2-3%
@ 85mg/dl

Accuracy - Recovery

Plasma; mean 100.9%
(99.2-106.7%)
range 1.5-30.0mmol/L
Whole blood; mean 96.7%
(89.1-100.2%)

Plasma; mean 99.2%
(96.0-104.0%)
range 2.5-10.0mmol/L
Whole blood; mean 96.7%
(88.0-104.0%)

Spiked serum;
y (Analox) = 0.973 (true)
+ 2.1mg/dl
r = 0.9929, n = 89

Accuracy - Method
Comparison,
y = GM7

Plasma vs Hexokinase
y = 0.96 (HK) - 2.5mg/dl
r = 0.999; n = 156 (Plasma)

Plasma vs Boehringer UV,
n = 20; r = 0.997
y = 0.967(UV) + 0.003
Whole blood vs YSI 23L,
n = 56, r = 0.998
y = 1.017(YSI) - 0.087

Whole blood vs GC,
y (Analox) = 1.04(GC)
+ 1.3mg/dl
r = 0.9905, n  = 27

Linearity

0 - 30mmol/L (540mg/dl)
using 10µl sample
0 - 50mmol/L (900mg/dl)
using 5µl sample

0 - 10mmol/L (90mg/dl)
using 7µl sample
0 - 20mmol/L (180mg/dl)
using 3.5µl sample

0 - 200mg/dl (43mmol/L)
using 5µl sample
0 - 400mg/dl (86mmol/L)
using 2.5µl sample

 

Instrument Specification:
 

Method
Sensor
Reaction temperature
Display
Printer

Enzymatic oxygen-rate
Clark-type amperometric oxygen electrode
30°C
32 character backlit LCD
16 column dot matrix, 1 line/sec 

Statistical programmes

a)Sequential, giving mean, S.D and C.V.
b) Selected results for 2 analytes, retains up to 75 entries

Interface
Power
Dimensions
Weight

Serial data port, optional Windows software available
100-250V AC, 50-60Hz, 12-15V DC, 60VA
27cm (width) x 35cm (depth) x 25cm (height)
8.4Kg

 

Ordering Information:
 

P-GM7 MicroStat
GM7 MicroStat
GMRD-002A
GMRD-011
GMRD-021
GMRD-084

Portable analyser, rechargeable battery/mains
Analyser, mains version
Glucose oxidase reagent, 360 analyser cycles
Glucose standard, 8mmol/L, 30ml (other values available)
Uric acid reagent kit, 70 analyser cycles incl. 0.6mmol/L standard
Cholesterol reagent kit, 50 tests, incl. 5.2mmol/L standard

GMRD-090/093/092

Lactate reagent for intact whole blood - 100, 250 and 1000 analyser
cycles respectively, including 8.0mmol/L standard

GMRD-100/103/102

Lactate reagent for lysed whole blood - 100, 250 and 1000 analyser
cycles respectively, including 8.0mmol/L standard

GMRD-140
GMRD-150
GMRD-162
GMRD-050
GMRD-053/054
GMRD-055/056

Pyruvate reagent kit, 20-40 tests, incl. standard
Glutamine reagent kit, 50 tests, incl. standard
Ammonia reagent kit, 20-40 tests, incl. standard
Record cards for collection capillaries, pack of 40
Blood collection capillaries, packs of 500 and 1000 respectively
Closures for collection capillaries, packs of 100 and 500 respectively

Specialised blood collection systems and accessories are available – please contact Analox for details