Technical Summary: 3-Hydroxybutyrate
Analysis
(Bulletin ref. TB12036)
A fast
enzymatic analysis for plasma 3-Hydroxybutyrate.
Name:
Code:
Size:
3-Hydroxybutyrate
GMRD-135
60 (3x20)
tests (140 analyser
cycles). Supersedes kit GMRD-135
Instruments:
All
GM7 Series analysers
Principle:
i) The
enzyme 3-hydroxybutyrate dehydogenase (3-HBDH) reduces NAD in proportion
to the 3-hydroxybutyrate concentration in a brief pre-reaction:
ii) The rate of oxidation
by peroxidase (POD) of the generated NADH is determined in the analyser
and under appropriate conditions is directly proportional
to the plasma 3-hydroxybutyrate concentration:
Samples:
Sample
volume:
Linearity:
Detection Limit:
Precision:
Plasma
(heparin), serum
50 µl
5000
µmol/L (52 mg/dl)
100
µmol/L (2mg/dl)
Typical within-run CV = 3 - 4 % @ 480
µmol/L (SD = 0.2mg/dl)
Accuracy:
Method
comparison
vs SIGMA enzymatic spectrophotometric method
y (Analox) = 0.95x - 0.04 mmol/L, r = 0.985;
n = 33
Reagent Stability:
Shelf-life 9 months unopened at 0-5°C. Tris/NAD/3-HBDH reagent 14 days at 0-5°C after reconstitution; 3 vials/kit. POD reagent 5-6 weeks at 0-5°C.
