Technical Summary: HDL Cholesterol Analysis (Bulletin ref. TB059030E)
 

Analox High Density Lipoprotein (HDL) Reagent is intended for the quantitative determination of the HDL cholesterol fraction in plasma or serum and hence its relationship (%) to the total plasma or serum cholesterol.

The inverse relationship between HDL concentration and cardiovascular disease risk is well established. Epidemiological studies have stressed the association of low levels of HDL cholesterol with increased risk of ischaemic heart and ischaemic cerebrovascular disease. Thus measurement of the HDL cholesterol fraction is useful in identifying individuals at increased risk.
 

Name:
Codes:

HDL Cholesterol Microtubes
GMRD-089 (50 ready-to-use, pre-dosed reagent tubes)
 

Instruments:
 

All GM7/GL5 Series analysers
 

Additional
Requirements:

Cholesterol Rate Reagent Kit (GMRD-084) or
Cholesterol End-Point Reagent Kit (GMRD-083)
Suitable Microcentrifuge (e.g. Analox MBC - see Note i) below)
 

Principle:

When plasma/serum is mixed with the lyophilised reagent, the dextran sulphate and magnesium ions present produce selective precipitation of low density lipoprotein fractions (LDL/VLDL). The HDL cholesterol fraction remains in the supernatant after high speed centrifugation. The supernatant HDL cholesterol concentration is measured and then compared to the plasma/serum total cholesterol.
 

Samples:
Sample volume:
Centrifuge Time:
 

Plasma or serum.
50 µl
1 minute (Analox MBC microcentrifuge)
 

Reagent Stability:

Shelf life 12 months at 0 - 5°C.
 

Notes:

i) The Analox MBC microcentrifuge is ideal for both plasma separation and LDL/VLDL precipitation. Plasma separation can be achieved in approximately one minute using Analox heparinised blood collection tubes, GMRD-067 (100/pk). The fill (0.5 ml) is appropriate for capillary site (fingerprick) blood sampling.
ii) HDL cholesterol concentration is measured after transferring approximately
30 µl of supernatant to the special capillaries in the GMRD-083 or GMRD-084 reagent kits and proceeding as directed in the respective instructions.