Technical Summary: Immediate Whole Blood Glucose Analysis - Glucose Direct
(Bulletin ref. TB010249)
 

An ultra fast enzymatic assay for haemolysed whole blood glucose in clinical or research applications, e.g. clamp experiments. Where appropriate, blood may be transferred from the sampling site directly to the analyser with no collection or pre-treatment system, providing the fastest analysis of samples as small as 2 µl.
 

Name:
Code:
Size:

Glucose Direct
GMRD-020
250 analyses (720 analyser cycles)
 

Instruments:
 

GM9D Glucose Direct analyser
 

Principle:

In the presence of molecular oxygen, glucose is oxidised by the enzyme glucose oxidase to gluconic acid and hydrogen peroxide:
 

Under the conditions of the assay, oxygen consumption is directly proportional to glucose concentration.
 

Samples:

Whole blood samples transferred directly from the sampling site or via common anticoagulation systems containing heparin, oxalate or EDTA. No special Analox collection vessels are required as all additives are contained within the analyser reagent system. Plasma, serum, C.S.F., urine (suitably diluted) and other aqueous media samples may also be used.
 

Sample volume:
Analysis time
 

2, 5 or 10 µl (variable 2 - 10 µl)
From 35 seconds
 

Linearity:

30 mmol/L (10 µl sample); 540 mg/dl
50 mmol/L (5 µl sample); 900 mg/dl
 

Precision:

Typical within-run C.V. = 2 % at 5 mmol/L (S.D. range 0.07 - 0.26)
 

Accuracy:

a) Recovery - Whole-blood, mean, 96.7 % (89.1 %-100.2 %)
range 1.0 - 25.0mmol/L
b) Method Comparison - Analox oxygen rate vs Hexokinase (Reference)
y (Analox) = 0.98x - 0.14 mmol/L, r = 0.999; n = 156 (serum)
 

Matrix Comparisons
on GM9D:

a) Plasma vs Whole Blood (rat),
r = 0.994, y (plasma) = 1.11x - 0.11 (mmol/L)
b) Plasma vs Whole Blood (human),
r = 0.998, y (plasma) = 1.06x - 0.61 (mmol/L)
 

Reagent Stability:

Shelf-life 18 months stored at 0 - 5°C.
 

Note:

GMRD-020 reagent kit includes a glucose standard at 8.0 mmol/L. Other values at 2.5, 5.0 and 25.0 mmol/L can be supplied separately.