Technical Summary: Industrial Glucose Analysis (Bulletin ref. TB010441C)
 

A very fast electrochemical enzymatic assay for ß-D-glucose (dextrose) in beverages, biotechnology and biopharmaceutical applications.
 

Name:
Code:
Size:

Glucose (Industrial)
GMRD-002A
250ml (144 analyser cycles)

Instruments:
 

GM10 Series analysers
 

Principle:

In the presence of molecular oxygen, glucose is oxidised by the enzyme glucose oxidase to gluconic acid and hydrogen peroxide:
 

Under the conditions of the assay, oxygen consumption is directly proportional to glucose concentration.
 

Samples:

Any suitable homogenous biological fluid or aqueous extract. Gross particulates should be removed by filtration or centrifugation. However, turbidity or opacity does not cause interference.
 

Sample volume:
Analysis time:

5µl-25 µl (depending on concentration)
20 seconds
 

Analytical cycle
Time:

Approx. 60 seconds
 

Concentration
Range:

0.05- 4%W/V (direct injection); 0 - 40 %W/V (with simple aqueous dilution)
 

Reagent Stability:

Shelf-life greater than 15 months stored at 0 - 5°C.

Note:

Long-life glucose standards (with preservative) at 1.0, 2.0, 5.0, 8.0 and 20.0 %W/V (30 ml) are available and ordered as required. Other values can be supplied to special order.