Technical Summary: Blood Glutamine Analysis (Bulletin ref. TB049742B)
 

A fast enzymatic analysis for plasma L-glutamine.
 

Name:
Code:
Size:

Blood Glutamine
GMRD-150
2 x 25 tests; 140 analyser cycles

Instruments:
 

All GM7 Series analysers
 

Principle:

i) Ammonia, either endogenous or liberated from L-glutamine by the enzyme glutaminase (GLU), is converted to L-glutamate by a-ketoglutarate in the presence of glutamate dehydrogenase (GLDH) and excess NADH in a brief pre-reaction:

ii) Under the conditions of the assay, the rate of oxidation of excess NADH by peroxidase (POD) is inversely proportional to the total ammonia present:

A sample blank without GLU measures endogenous ammonia and other NADH consuming reactions and the L-glutamine concentration is obtained by difference.
 

Samples:
Sample volume:
Linearity:
Precision:
 

Plasma
100 µl (2x50 µl)
2000 µmol/L (30 µg/dl)
Typical within-run S.D. = 10 µmol/L, C.V. = 1 - 2 % @ 600 µmol/L

Reagent Stability:

Shelf-life 9 months unopened when stored at 0 - 5°C. Reconstituted
GLDH/NADH/a-ketoglutarate, 7 - 10 days (2 vials) if stored at 0 - 5°C.
 

Note:

For industrial L-glutamine/ammonia analysis in industrial (e.g. biotechnology)
applications, please refer to details of product GMRD-151 which is intended
for concentrations up to 20 mmol/L