Technical Summary: Clinical Glycerol Analysis (Bulletin ref. TB080148)
 

A simple, rapid and direct enzymatic analysis for glycerol in plasma or serum.
 

Name:
Code:
Size:
Instruments:
 

Glycerol II Assay
GMRD-177
100ml (140 analyser cycles)
GM7 Series analysers
 

Principle:

In the presence of glycerol kinase (GK), glycerol is phosphorylated by adenosine triphosphate (ATP) forming glycerol-3-phosphate (G-3-P) which in turn is oxidised by glycerol-3-phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide:
 

i)

ii)

Under the conditions of the assay, reactions i) and ii) run concurrently in
the reaction chamber and the rate of oxygen consumption is directly
proportional to plasma glycerol concentration.
 

Samples:
Sample Volume:
Calibration Value:
 

Serum or plasma containing heparin, EDTA or oxalate anticoagulants
25 µl
0.50 mmol/L, 500 µmol/L, 4.5 mg/dl
 

Analytical Range:
Analysis Time:

0.2 - 1.0 mmol/L, ca 2 - 10 mg/dl
20 - 25 seconds

Analytical Cycle Time:
Precision:
Analyser Sensitivity:

Reagent Stability:

Approx. 60 seconds
Typical S.D. = 0.05 at 5 mg/dl (within run)
0.01 mmol/L; 0.1 mg/dl

Shelf-life 12 months unopened when stored at 0 - 5°C,
3 - 4 weeks after reconstitution stored at 0 - 5°C between use.
 

Note:

Levels below ca 0.2 mmol/L (2 mg/dl) can be regarded as clinically normal for human plasma. Signal response below this level may be indistinguishable from instrument 'noise' due to sample injection.