Technical Summary: Pyruvate Analysis (Bulletin ref. TB04867B)
 

A fast enzymatic analysis for plasma pyruvate.
 

Name:
Code:
Size:

Pyruvate
GMRD-140
20 tests; 140 analyser cycles. The number of tests may be doubled by using half-volumes (micro-method).
 

Instruments:
 

All GM7 Series analysers
 

Principle:

i) In the presence of excess NADH, endogenous plasma lactate dehydrogenase (LDH) rapidly converts pyruvate to lactate in a brief pre-reaction:

ii) Under the conditions of the assay, the rate of oxidation of excess NADH by peroxidase (POD) is inversely proportional to the pyruvate concentration:

A sample blank allows for background interference.
 

Samples:
Sample volume:
Analytical Range:
Precision:
 

Plasma
100 µl
1000 µmol/L (8.9 mg/dl)
Typical within-run C.V. = 5 % @ 200 µmol/L
 

Accuracy:

Method comparison vs Spectrophotometric UV (Sigma) procedure:
y (Spectro) = 0.97 (Analox) - 0.1 mg/dl, r = 0.940, n = 46
 

Reagent Stability:

Shelf-life 9 months unopened when stored at 0 - 5°C. Reconstituted NADH
reagent and standard, 10 days if stored at 0 - 5°C.