Technical Summary: Triglycerides Analysis (Bulletin ref. TB110143)
 

A simple, rapid enzymatic analysis for plasma or serum triglycerides.
 

Name:
Code:
Size:

Plasma Triglycerides Assay
GMRD-195
100ml (140 analyser cycles)
 

Instruments:

All GM7 Series analysers
 

Principle:

i) Plasma triglycerides are hydrolysed to glycerol and fatty acids in a rapid pre-reaction with Lipase (LP):
 

In the presence of glycerol kinase (GK), glycerol is phosphorylated by adenosine triphosphate (ATP) forming glycerol-3-phosphate (G-3-P) which in turn is oxidised by glycerol-3-phosphate oxidase (GPO) to dihydroxyacteone phosphate (DAP) and hydrogen peroxide:
 

ii)

iii)

Under the conditions of the assay, reactions i) and ii) run concurrently in the reaction chamber and the rate of oxygen consumption is directly proportional to the plasma triglyceride concentration.
 

Samples:
Sample Volume:
Injection Volume:
Calibration Value:
Linearity:
Analysis Time:
Precision:
Analyser Sensitivity:
 

Serum or plasma containing heparin, EDTA or oxalate anticoagulants
25 µl
20 µl
2.26 mmol/L, 200 mg/dl (Triolein)
5 mmol/L (10 mmol/L using 10 µl)
20 - 25 seconds (from injection)
Typical within-run S.D. = 0.02 @ 2 mmol/L
0.01 mmol/L, 0.1 mg/dl
 

Reagent Stability:

Shelf-life 9 months unopened when stored at 0 - 5°C. 3 - 4 weeks after reconstitution when stored at 0 - 5°C between use.