Technical Summary: Uric Acid Analysis (Bulletin ref. TB120034C)
 

A very fast, simple enzymatic assay for plasma uric acid using Uricase.
 

Name:
Code:
Size:

Uric Acid
GMRD-021
50ml (70 analyser cycles).

Instruments:
 

All GM7 and GL5 Series analysers
 

Principle:

In the presence of molecular oxygen, uric acid is oxidised by the enzyme uricase (urate oxidase) to allantoin and hydrogen peroxide:
 

Under the conditions of the assay, oxygen consumption is directly proportional to uric acid concentration:
 

Samples:
Sample volume:
Analysis time:
Linearity:
Precision:
 

Plasma, serum
10 µl
20 seconds
0.80 mmol/L (13.3 mg/dl)
Typical within-run S.D. = 0.01 mmol/L
 

Accuracy:

i) Recovery data 94 - 108 %
ii) Correlation: y (Analox) = 1.022(AAII) + 0.004 mmol/L,
r = 0.965; n = 53
 

Reagent Stability:

Shelf-life 6 months unopened when stored at 0 - 5°C. Enzyme/buffer reagent
2 - 3 weeks after reconstitution if stored at 0 - 5°C between use.