A fast and precise enzymatic assay for plasma cholesterol using Cholesterol Oxidase.
Our Clinical & Research is explained in detail below.
i) Cholesterol esters are converted to free cholesterol in a brief pre-reaction:
ii) In the presence of molecular oxygen, cholesterol is oxidised by the enzyme cholesterol oxidase to cholestenone and hydrogen peroxide
Under the conditions of the assay, oxygen consumption is directly proportional to cholesterol concentration.
|Samples||Plasma or serum. Whole blood for screening only|
|Sample Volume||30 µl|
|Analysis Time||20 seconds|
|Analytical Cycle||Approx. 60 seconds|
0-10 mmol/L (0-387 mg/dl)
|Precision||C.V. of 1 % @ 5 mmol/L (194 mg/dl)|
|Detection Limit||0.25 mmol/L (ca. 10 mg/dl)|
Shelf-life unopened: 6 months stored at 0 - 5°C.
Shelf-life reconstituted: Cholesterol oxidase enzyme, 7 - 10 days stored at 0 - 5°C.
Whole blood cholesterol values are lower than plasma or serum by on average 15 - 20 %.
Analysis of High Density Lipoprotein (HDL) cholesterol fraction can be performed using Analox HDL microtubes, code: GMRD-089