Ammonia
Ammonia is usually measured in biotechnology, pharmaceutical and fermentation and culture control applications
Industry & Biotechnology
Our Industry & Biotechnology is explained in detail below.
Industry & Biotechnology
A simple and rapid enzymatic analysis for ammonia in a variety of biological fluids, e.g. in monitoring and control of culture media and fermentation processes in biotechnology industries.
Principle
i) Ammonia is converted to L-glutamate by glutamate dehydrogenase (GLDH) in the presence of a-ketoglutarate and excess NADH in a brief pre-reaction:
ii) Under the conditions of the assay, the rate of oxidation of the excess NADH by peroxidase (POD) is inversely proportional to the total ammonia present:
Technical Summary
| Samples | Aqueous solutions (pH 5 - 7), culture fluid, etc. |
| Sample Volume | 25 µl |
| Analysis Time | 20 seconds (from injection) |
| Analytical Range | Typical within-run C.V. = 1 - 2 % @ 8.0 mmol/L |
| Linearity | N/A |
| Precision | Typical within-run C.V. = 1 - 2 % @ 8.0 mmol/L |
| Detection Limit | 0.2 mmol/L |
| Reagent Stability | Shelf-life unopened: 12 months stored at 0-5°C. Shelf-life reconstituted: NADH/a- ketoglutarate, 7 - 10 days stored at 0-5°C. |
Notes:
A sample blank allows for interference by endogenous NADH consuming reactions.
Analysers
No analysers are currently available for this analyte.
