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 Ammonia  is usually measured in biotechnology, pharmaceutical and fermentation and culture control applications

Industry & Biotechnology

Our Industry & Biotechnology is explained in detail below.

Industry & Biotechnology

A simple and rapid enzymatic analysis for ammonia in a variety of biological fluids, e.g. in monitoring and control of culture media and fermentation processes in biotechnology industries.


i) Ammonia is converted to L-glutamate by glutamate dehydrogenase (GLDH) in the presence of a-ketoglutarate and excess NADH in a brief pre-reaction:

ii) Under the conditions of the assay, the rate of oxidation of the excess NADH by peroxidase (POD) is inversely proportional to the total ammonia present:

Technical Summary

Samples Aqueous solutions (pH 5 - 7), culture fluid, etc.
Sample Volume 25 µl
Analysis Time 20 seconds (from injection)
Analytical Range Typical within-run C.V. = 1 - 2 % @ 8.0 mmol/L


Precision Typical within-run C.V. = 1 - 2 % @ 8.0 mmol/L
Detection Limit 0.2 mmol/L
Reagent Stability

Shelf-life unopened: 12 months stored at 0-5°C. 

Shelf-life reconstituted: NADH/a- ketoglutarate, 7 - 10 days stored at 0-5°C.


A sample blank allows for interference by endogenous NADH consuming reactions.


No analysers are currently available for this analyte.

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