Ammonia is usually measured in biotechnology, pharmaceutical and fermentation and culture control applications
Our Industry & Biotechnology is explained in detail below.
A simple and rapid enzymatic analysis for ammonia in a variety of biological fluids, e.g. in monitoring and control of culture media and fermentation processes in biotechnology industries.
i) Ammonia is converted to L-glutamate by glutamate dehydrogenase (GLDH) in the presence of a-ketoglutarate and excess NADH in a brief pre-reaction:
ii) Under the conditions of the assay, the rate of oxidation of the excess NADH by peroxidase (POD) is inversely proportional to the total ammonia present:
|Samples||Aqueous solutions (pH 5 - 7), culture fluid, etc.|
|Sample Volume||25 µl|
|Analysis Time||20 seconds (from injection)|
|Analytical Range||Typical within-run C.V. = 1 - 2 % @ 8.0 mmol/L|
|Precision||Typical within-run C.V. = 1 - 2 % @ 8.0 mmol/L|
|Detection Limit||0.2 mmol/L|
Shelf-life unopened: 12 months stored at 0-5°C.
Shelf-life reconstituted: NADH/a- ketoglutarate, 7 - 10 days stored at 0-5°C.
A sample blank allows for interference by endogenous NADH consuming reactions.