Sucrose can be measured in the Industrial & Biotechnology applications.
Please see examples below.
Sucrose is usually measured in the fermentation and culture control applications
A simple two-step electrochemical enzymatic assay for sucrose in aqueous solutions or extracts
i) Sucrose, a disaccharide, is stoichiometrically hydrolysed by Invertase (ß-fructosidase) to a-D-glucose and fructose in a simple pre-reaction. Under the special buffer conditions used, mutarotation to ß-D-glucose rapidly occurs:
|Samples||Aqueous solutions and extracts|
|Analysis Time||20 seconds (analyser)|
|Analytical Range||0.5 - 20 %W/V All analysers|
Shelf-life 9 months unopened stored at 0 - 5°C. Reconstituted invertase, 3 - 4 days (4 vials) if stored at 0 - 5°C. Aliquots may be frozen for extended life.
4 vials of Invertase (ß-fructosidase) are provided to maximise kit life.
Sample opacity or turbidity presents no problem since the sensor system is electrochemical rather than spectrophotometric.
Endogenous glucose, if present, should be determined as a sample 'blank', i.e. extract diluted pro-rata in water instead of ß-fructosidase.
Incomplete hydrolysis may take place for sucrose concentrations greater than 10 %W/V. For greater accuracy at these levels repeat hydrolysis using a 5µl sample and scale results as detailed in kit instructions.