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Analytes

Lactate

Lactate can be measured in both Clinical Research and Industrial, Commercial & Biotechnology applications

Please see examples below.

Clinical & Research

Lactate can be measured in Clinical Pathology, Sports Science and Exercise Physiology.

Industry & Biotechnology

Lactate can be measured in biotechnology, pharmaceutical and food & beverage applications.

Clinical & Research

A rapid high performance analysis for lactate in whole blood, plasma or serum, based on the direct reaction with Lactate Oxidase (LOD).

Principle

In the presence of molecular oxygen, lactate is oxidised by the enzyme Lactate Oxidase to pyruvate and hydrogen peroxide:

Under the conditions of the assay, oxygen consumption is directly proportional to the L-lactate concentration

Technical Summary

Samples Intact whole-blood and lysed whole-blood (collected in Analox tubes or capillaries), plasma, neutralised perchloric acid extracts and other aqueous media.
Sample Volume 5 µl or 7µl
Analysis Time 20 seconds
Linearity

0 -10 mmol/L, (0 - 90mg/dl) - 7 µl sample

0- 14 mmol/L, (0 - 126 mg/dl) - 5 µl sample

Precision C.V. of 2% @ 2.5 mmol/L
Detection Limit 0.3mmol/L @7 µl sample: 0.1mmol/L @ 25 µl sample
Reagent Stability

Shelf-life unopened: 12 months stored at 0-5°C. 

Shelf-life reconstituted: 60 days stored at 0-5°C.


Notes:

Quality Control Serum and sample record cards for attachment and identification of capillaries are available.

Lactate methodology has excellent precision at all levels, stable calibration, and is remarkably free from interferences.

Alternative standards at 3.0 and 5.0 mmol/L, and a quality control material, are available in addition to the kit calibrant of 8.0 mmol/L (72.1 mg/dl).

Industry & Biotechnology

A rapid high performance analysis based on the direct reaction with the specific enzyme L-Lactate Oxidase (LOD).

Principle

In the presence of molecular oxygen, lactate is oxidised by the enzyme lactate oxidase to pyruvate and hydrogen peroxide:

Under the conditions of the assay, oxygen consumption is directly proportional to the L-lactate concentration:

Technical Summary

Samples Aqueous or semi-aqueous samples such as dairy products, e.g. milk, can be used directly.
Sample Volume 5µl
Analysis Time 20 seconds
Analytical Range 0.006 - 14 mmol/L (ca. 0.5 - 126 mg/dl)
Precision Typical within-run C.V. 2 % @ 2.5 mmol/L
Reagent Stability

Shelf-life unopened: 12 months stored at 0-5°C. 

Shelf-life reconstituted: 60 days stored at 0-5°C.


Notes:

Turbidity or opacity is not a problem as the detection method is electrochemical rather than optical. 

Specimens should be in the pH range 4-8 and free from overt bacterial contamination.

Lactate methodology has excellent precision at all levels, stable calibration, and is remarkably free from interferences.

Alternative standards at 3.0 and 5.0 mmol/L, and a quality control material, are available in addition to the kit calibrant of 8.0 mmol/L (72.1 mg/dl).

The enzyme lactate oxidase is 100 % specific to L-Lactate. D-lactate is not measured.

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