Triglycerides can be measured in the Clinical Research applications.
Please see examples below.
Triglycerides are normally measured in clinical pathology applications
A simple, rapid enzymatic analysis for plasma or serum triglycerides.
Plasma triglycerides are hydrolysed to glycerol and fatty acids in a rapid pre-reaction with Lipase (LP):
In the presence of glycerol kinase (GK), glycerol is phosphorylated by adenosine triphosphate (ATP) forming glycerol-3-phosphate (G-3-P) which in turn is oxidised by glycerol-3-phosphate oxidase (GPO) to dihydroxyacteone phosphate (DAP) and hydrogen peroxide:
Under the conditions of the assay, reactions ii) and iii) run concurrently in the reaction chamber and the rate of oxygen consumption is directly proportional to the plasma triglyceride concentration
|Samples||Serum or plasma containing heparin, EDTA or oxalate anticoagulants|
|Sample Volume||25 µl|
|Analysis Time||20 - 25 seconds (from injection)|
0 - 5 mmol/L (ca. 0 - 440 mg/dl)
Shelf-life unopened: 9 months stored at 0 - 5°C.
Shelf-life reconstituted: 3 - 4 weeks stored at 0 - 5°C.