Skip to main content
Analytes

Triglycerides

Triglycerides can be measured in the Clinical Research applications.

Please see examples below.

Clinical & Research

Triglycerides are normally measured in clinical pathology applications

Clinical & Research

A simple, rapid enzymatic analysis for plasma or serum triglycerides.

Principle

Plasma triglycerides are hydrolysed to glycerol and fatty acids in a rapid pre-reaction with Lipase (LP):

i)

In the presence of glycerol kinase (GK), glycerol is phosphorylated by adenosine triphosphate (ATP) forming glycerol-3-phosphate (G-3-P) which in turn is oxidised by glycerol-3-phosphate oxidase (GPO) to dihydroxyacteone phosphate (DAP) and hydrogen peroxide:

ii)

iii)


Under the conditions of the assay, reactions ii) and iii) run concurrently in the reaction chamber and the rate of oxygen consumption is directly proportional to the plasma triglyceride concentration

Technical Summary

Samples Serum or plasma containing heparin, EDTA or oxalate anticoagulants
Sample Volume 25 µl
Analysis Time 20 - 25 seconds (from injection)
Linearity

0 - 5 mmol/L (ca. 0 - 440 mg/dl)

Reagent Stability

Shelf-life unopened: 9 months stored at 0 - 5°C. 

Shelf-life reconstituted: 3 - 4 weeks stored at 0 - 5°C.


Notes:

Newsletter Sign Up

If you would like to be kept informed of the work and news of Analox, please subscribe to our newsletter.