Triglycerides

Triglycerides can be measured in the Clinical Research applications.

Please see examples below.

Clinical & Research

Triglycerides are normally measured in clinical pathology applications

Clinical & Research

A simple, rapid enzymatic analysis for plasma or serum triglycerides.

Principle

Plasma triglycerides are hydrolysed to glycerol and fatty acids in a rapid pre-reaction with Lipase (LP):

i)

In the presence of glycerol kinase (GK), glycerol is phosphorylated by adenosine triphosphate (ATP) forming glycerol-3-phosphate (G-3-P) which in turn is oxidised by glycerol-3-phosphate oxidase (GPO) to dihydroxyacteone phosphate (DAP) and hydrogen peroxide:

ii)

iii)

Under the conditions of the assay, reactions ii) and iii) run concurrently in the reaction chamber and the rate of oxygen consumption is directly proportional to the plasma triglyceride concentration